Volume 4 Supplement 1

Proceedings of the Second International Cilia in Development and Disease Scientific Conference (2014)

Open Access

Comparative genomics reveals novel genes associated with sensory cilia

  • B Piasecki1,
  • T Sasani1,
  • B O'Flaherety1,
  • T Swords1,
  • J Henriksson2,
  • E De Stasio1 and
  • P Swoboda2
Cilia20154(Suppl 1):P16

DOI: 10.1186/2046-2530-4-S1-P16

Published: 13 July 2015

Objective

To identify evolutionarily conserved ciliary genes that facilitate sensory-specific roles.

Methods

Using reciprocal BLAST analyses, the genomes of organisms that do not make cilia (the plant Arabidopsis thaliana and the yeast Saccharomyces cerevisiae) and that retain motile but not sensory cilia (the moss Physcomitrella patens) were subtracted from the genomes of organisms that have retained sensory cilia (the worm Caenorhabditis elegans and the alga Chlamydomonas reinhardtii).

Results

These analyses revealed a list of 272 genes that are found exclusively in organisms with sensory cilia but not motile cilia. Importantly, nearly 10% of the genes on this list have previously been implicated in sensory cilia-specific roles, thus providing numerous internal positive controls that demonstrate this list is enriched with sensory-specific ciliary genes. A subset of uncharacterized candidate genes are currently being studied in C. elegans, which retains cilia exclusively on a set of neurons termed ciliated sensory neurons (CSNs). We are currently generating a number of promoter- and gene-to-green fluorescent protein (GFP) fusion constructs in order to determine the expression and localization patterns of the proteins encoded by these genes. Two of these candidate genes, which are found in worms (C. elegans) and algae (C. reinhardtii) but not in moss (P. patens) have been termed wam-1 and wam-2, respectively. Expression of wam-1 appears to be localized exclusively in the support cells of ciliated dopaminergic neurons, while expression of wam-2 has yet to be fully characterized.

Conclusion

Our analyses have successfully revealed novel genes involved in ciliary sensory-specific processes in animals.

Authors’ Affiliations

(1)
Biology, Lawrence University
(2)
Department of Biosciences and Nutrition, Karolinska Institute

Copyright

© Piasecki et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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