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Figure 2 | Cilia

Figure 2

From: Development of a method for the measurement of primary cilia length in 3D

Figure 2

2D maximum projections (2D MP) significantly underestimate microfiber length in 3D. (A) 2D image of the 50 μm microfibers set in their original preparation. (B) Mean microfiber (± SD) lengths measured from wide-field fluorescence (WF) or confocal laser scanning microscopy (CLSM) 2D maximum projection images of fibers in 3D constructs. MP measurements from both WF and CLSM data were significantly different from the original population mean. There was no difference in length measurements obtained from MP images of WF and CLSM data (**P ≤ 0.01).

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