Figure 3
From: Development of a method for the measurement of primary cilia length in 3D
Creating a spherical point spread function (PSF). The discrepancy between axial and lateral resolution is illustrated by the shape of the PSF in the xy and xz optical planes. The combination of deconvolution (Decon) and Gaussian blurring (GB) equalizes the axial and lateral resolutions. This results in a PSF that is approximately spherical and therefore has the same size and shape in both xy and xz planes. Scale bar = 1 μm.