Figure 4
From: Development of a method for the measurement of primary cilia length in 3D
The combination of deconvolution and Gaussian blurring restores object morphology. Pseudocolored 2D maximum projection images of a 4 μm microsphere in the xy and xz plane. The combination of deconvolution (Decon) and Gaussian blurring (GB) rectifies the optical distortion in the images so that the microspheres appear spherical in both xy and xz. Scale bar = 2 μm.