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Table 1 Making the point spread function (PSF) spherical

From: Development of a method for the measurement of primary cilia length in 3D

 

Raw

Deconvolved

Deconvolved and Gaussian blurred

xy (nm)

540 ± 76.4

324 ± 76.4

1,080 ± 76.4

xz (nm)

2,000 ± 176.8

1,000 ± 176.8

1,000 ± 176.8

Ratio xy:xz

0.27 ± 0.01

0.32 ± 0.02

1.08 ± 0.02

  1. Full-width-half-maximum (FWHM) measurements of experimental PSFs for raw, deconvolved and deconvolved and Gaussian blurred 3D images (n = 5).
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Cilia

ISSN: 2046-2530

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