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Table 2 Restoration of microsphere morphology

From: Development of a method for the measurement of primary cilia length in 3D

 

Raw

Deconvolved

Deconvolved and Gaussian blurred

xy (μm)

4.46 ± 0.28

3.16 ± 0.18

4.03 ± 0.31

xz (μm)

5.51 ± 0.65

4.01 ± 0.46

4.02 ± 0.46

Ratio xy:xz

0.82 ± 0.18

0.80 ± 0.11

1.02 ± 0.14

  1. Diameter of 4 μm microspheres (mean ± SD) measured from binary images of xy and xz 2D maximum projections of raw, deconvolved and deconvolved and Gaussian blurred 3D images (n = 17).