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Figure 2 | Cilia

Figure 2

From: Ciliary and non-ciliary expression and function of PACRGduring vertebrate development

Figure 2

PACRG expression in the developing Xenopus brain. (A) Brain anatomy of the 3-day tadpole (stage 45), as highlighted by rhodamine dextran injection into the hindbrain ventricle. (B) Isolated brain following in situ hybridization with an antisense PACRG probe, shown in dorsal (left) and ventral (right) view. (C) Whole-mount of brain in which the hindbrain was removed and a dorsal cut was introduced between forebrain and interbrain to expose the choroid plexus (higher magnification shown in inset). (D-G) Transversal histological sections; levels indicated in (B), dorsal side up. (D) Staining in the thalamic nuclei. (E) Localized expression in roof of cerebral aqueduct (cilia highlighted by arrowheads in the enlargement shown in (E’)). (F) Expression in ventral midline of midbrain region. (G) Staining in roof of hindbrain. (H) Scanning electron micrograph, demonstrating multiciliated cells in roof of hindbrain. ca = cerebral aqueduct; cp = choroid plexus; fb = forebrain; hb = hindbrain; ib = interbrain; mb = midbrain; tn = thalamic nucleus. Note that dark brown spots in whole-mount brain specimens (B) and sections (D-G) represent melanocytes.

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