PACRG protein localization. (A-D) Immunohistochemistry. PACRG shown in green and acetylated tubulin in red. (A) Ventral view of gastrocoel roof plate (GRP) (dashed white line) at stage 17. (A’) Enlargement of GRP cilia. (B) Epidermis. Overview and schematic depiction of multiciliated epidermal cell. (C,D) Enlargements of cilia (C) and cytoplasmic PACRG staining (D). Note that within the cell PACRG localizes to vesicle-like structures and the perinuclear region. (E,F) Expression of a PACRG-enhanced green fluorescent protein (PACRG-eGFP) fusion protein. (E) Maximum intensity projection of timelapse movie of stage 17 GRP cilia, demonstrating elliptic shape of ciliary beating. (F) Squash preparation and (F’) maximum intensity projection of timelapse movie of stage 25 multiciliated skin cell. a = anterior; ac = acetylated; l = left; p = posterior; r = right.