Figure 2From: ciliaFA: a research tool for automated, high-throughput measurement of ciliary beat frequency using freely available softwareData analysis of one. AVI file of ependymal cilia before (A), and 15 minutes (B) after the addition of the bacterial toxin, pneumolysin. Images show (i) the area of brain tissue investigated, (ii) the regions of interest (ROI) used for analysis (color coding shows the intensity of the ciliary beat frequency (CBF) for each ROI: the darker the displayed color, the higher the CBF) and (iii) a histogram of the CBF of all regions of interest.Back to article page