Figure 2

Characterization of cell cultures of ovarian surface epithelium. A) Light microscopy (LM) images of wt and cancer OSE cells in cultures at sub-confluent and confluent stages. Anti-α-tubulin (α-tub) and phalloidin (F-actin) were applied to vizualize the cytoskeletal microtubules and actin, respectively, by IFM analysis, and anti-cytokeratin 8 (CK8) and anti-cytokeratin 18 (CK18) were applied to visualize the keratin components characteristic of human OSE cells. B) IFM analysis with primary antibodies against markers differentially expressed in wt and cancer OSE cells. See text for details. DNA was stained with DAPI. C) WB of wt and cancer OSE cells showing their expression of proteins characteristic for human OSE cells. EB1 was applied as loading control. DAPI, 4′,6-diamidino-2-phenylindole; IFM, immunofluorescence microscopy; OSE, ovarian surface epithelium; WB, western blot; wt, wild type.