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Table 1 Primary antibodies applied in this study

From: Primary cilia and aberrant cell signaling in epithelial ovarian cancer

Antigen IgG Source IHC IFM WB
Acetylated α-tubulin mMouse Sigma 1:1000 1:5000 1:2500
Aurora A/AlK (1 G4) mRabbit Cell Signaling   1:100 1:200
Centrin-2 (N-17) pGoat Santa Cruz   1:500  
CHFR pRabbit This study   1:500 1:10.000
Cytokeratin-8 (M-20) mMouse Santa Cruz 1:100 1:100  
Cytokeratin-18 mRabbit Epitomics 1:100 1:100 1:1500
Detyrosinated α-tubulin pRabbit Abcam 1:800 1:800  
EB1 (KT51) mRat Absea    1:1000
EB3 (KT36) mRat Absea   1:200  
E-cadherin pRabbit Cell Signaling 1:25 1:25 1:200
GFP mMouse Abcam   1:200 1:1000
IFT20 pRabbit kind gift from Greg Pazour   1:1000 1:1000
IFT88 pRabbit kind gift from Greg Pazour   1:1000 1:1000
Ki67 pRabbit Abcam   1:500  
N-cadherin pRabbit Abcam 1:200 1:200 1:800
Patched-1 pGoat Santa Cruz   1:200  
PCNA (PC10) mMouse Cell Signaling    1:2000
Pericentrin (C-16) pGoat Santa Cruz   1:200  
Phospho-RB (Ser807/811) pRabbit Cell Signaling    1:200
Smoothened pRabbit Abcam   1:100  
GLI2 (G-20) pGoat Santa Cruz    1:200
GLI2 (H-300) pRabbit Santa Cruz   1:100  
Vimentin mMouse Abcam 1:100 1:100 1:500
α-tubulin mMouse Sigma   1:500 1:2000
β-actin mMouse Sigma    1:10.000