Table 1 Primary antibodies applied in this study
From: Primary cilia and aberrant cell signaling in epithelial ovarian cancer
Antigen | IgG | Source | IHC | IFM | WB |
|---|---|---|---|---|---|
Acetylated α-tubulin | mMouse | Sigma | 1:1000 | 1:5000 | 1:2500 |
Aurora A/AlK (1 G4) | mRabbit | Cell Signaling |  | 1:100 | 1:200 |
Centrin-2 (N-17) | pGoat | Santa Cruz | Â | 1:500 | Â |
CHFR | pRabbit | This study | Â | 1:500 | 1:10.000 |
Cytokeratin-8 (M-20) | mMouse | Santa Cruz | 1:100 | 1:100 | Â |
Cytokeratin-18 | mRabbit | Epitomics | 1:100 | 1:100 | 1:1500 |
Detyrosinated α-tubulin | pRabbit | Abcam | 1:800 | 1:800 |  |
EB1 (KT51) | mRat | Absea | Â | Â | 1:1000 |
EB3 (KT36) | mRat | Absea | Â | 1:200 | Â |
E-cadherin | pRabbit | Cell Signaling | 1:25 | 1:25 | 1:200 |
GFP | mMouse | Abcam | Â | 1:200 | 1:1000 |
IFT20 | pRabbit | kind gift from Greg Pazour | Â | 1:1000 | 1:1000 |
IFT88 | pRabbit | kind gift from Greg Pazour | Â | 1:1000 | 1:1000 |
Ki67 | pRabbit | Abcam | Â | 1:500 | Â |
N-cadherin | pRabbit | Abcam | 1:200 | 1:200 | 1:800 |
Patched-1 | pGoat | Santa Cruz | Â | 1:200 | Â |
PCNA (PC10) | mMouse | Cell Signaling | Â | Â | 1:2000 |
Pericentrin (C-16) | pGoat | Santa Cruz | Â | 1:200 | Â |
Phospho-RB (Ser807/811) | pRabbit | Cell Signaling | Â | Â | 1:200 |
Smoothened | pRabbit | Abcam | Â | 1:100 | Â |
GLI2 (G-20) | pGoat | Santa Cruz | Â | Â | 1:200 |
GLI2 (H-300) | pRabbit | Santa Cruz | Â | 1:100 | Â |
Vimentin | mMouse | Abcam | 1:100 | 1:100 | 1:500 |
α-tubulin | mMouse | Sigma |  | 1:500 | 1:2000 |
β-actin | mMouse | Sigma |  |  | 1:10.000 |