Figure 1
From: A method for measuring electrical signals in a primary cilium
Culture system for the growth of ciliated cells on beads. (A-B) Phase-contrast image of live cells on a bead with contrast set to show cell bodies (A) or cilia (B). (B inset) Enlargement of cilium indicated by arrow. (C) Fluorescent image of bead from A and B labeled with di-8-ANEPPS (di-8). (C inset) Enlargement of cilium indicated by arrow. (D) Fluorescent image of a cell-coated bead fixed and immunolabeled for the ciliary marker acetylated α-tubulin (acet. tub.). (D inset) Enlargement of cilium indicated by arrow. (E) Teflon chamber with medium. Bar = 10 μm (A-D), 2 μm (B-D inset), 1 cm (E).