Colocalization of sperm-associated antigen (Spag)5 and isoform B of Ush2a (Ush2aisoB) and ninein-like (NINLisoB) proteins in the retina of young adult (P20) rat. Co-immunostaining of Spag5 and Ush2aisoB in retinal cryosections using (A) anti-SPAG5 antibodies (red) and (B) anti-USH2AisoB antibodies (green) show (C) partial colocalization (yellow) at the basal body. Co-immunostaining using (D) anti-SPAG5 antibodies (green) and (E) anti-NINLisoB antibodies (red). (F) A partial colocalization (yellow) was detected at the basal body. In addition, both antibodies labeled (Di,Ei) the ciliary rootlets, which was confirmed by (I) co-staining (yellow) using (G) anti-NINLisoB (green) and (H) anti-rootletin (red) antibodies.