Clusterin associated protein 1 (Cluap1) knockout mice are embryonic lethal. (A) Schematic of the wild-type Cluap1 allele (Cluap1WT) and the Cluap1 knockout allele (Cluap1KO). The relative position of the β-galactosidase cassette is indicated by the blue box. (B) PCR genotyping of Cluap1WT, Cluap1Het, and Cluap1KO embryos. (C) At E9.5, Cluap1KO embryos are runted, have enlarged pericardial sacs (arrow), and fail to turn properly (asterisk). (D) RT-PCR gel showing the expression of Cluap1 transcript in both Cluap1WT and Cluap1Het embryos and the absence in Cluap1KO embryos. Actin served as a positive template control in all samples. Reactions treated with reverse transcriptase (“+”) are alongside negative RT control samples (“- “). (E) Loss of the wild-type Cluap1 protein in Cluap1KO embryos was determined by Western blot. Actin was used as a loading control.