Figure 1

Clusterin associated protein 1 (Cluap1) knockout mice are embryonic lethal. (A) Schematic of the wild-type Cluap1 allele (Cluap1^WT) and the Cluap1 knockout allele (Cluap1^KO). The relative position of the β-galactosidase cassette is indicated by the blue box. (B) PCR genotyping of Cluap1^WT, Cluap1^Het, and Cluap1^KO embryos. (C) At E9.5, Cluap1^KO embryos are runted, have enlarged pericardial sacs (arrow), and fail to turn properly (asterisk). (D) RT-PCR gel showing the expression of Cluap1 transcript in both Cluap1^WT and Cluap1^Het embryos and the absence in Cluap1^KO embryos. Actin served as a positive template control in all samples. Reactions treated with reverse transcriptase (“+”) are alongside negative RT control samples (“- “). (E) Loss of the wild-type Cluap1 protein in Cluap1^KO embryos was determined by Western blot. Actin was used as a loading control.