Cluap1 is expressed in ciliated cells with a wide tissue distribution. (A) RT-PCR gel showing expression of Cluap1 in the indicated tissues; Sk. Muscle, skeletal muscle. Actin is used as a positive control. Reactions treated with reverse transcriptase (“+”) are alongside negative RT control samples (“- ”). (B) β-Galactosidase staining assay showing Cluap1 expression in Cluap1Het tissue in the ventricles of the heart, cortex of the kidney, lung tissue, and whole E9.5 embryo. Cluap1WT control tissue samples. Heart and kidney sections were counterstained in nuclear fast red. Scale bars are 10 μm in heart sections, 30 μm in kidney sections, and 1,000 μm for whole lung tissues and embryos.