Cluap1KOembryos fail to form primary cilia. (A,C,E) Cluap1WT E9.5 embryos were immunolabeled for the cilia marker acetylated α-tubulin (red) and Cluap1 (green) in the lateral plate mesenchyme of Cluap1WT embryos. (B,D,F) Cluap1KO embryos show a total loss of cilia in the same region. Hoechst nuclear stain in blue. Scale bar is 31.5 μm.