Cluap1KOembryos have downregulated expression of Patched-1 and Gli1. Real-time PCR results for the expression of Patched-1 and Gli1 in E9.5 Cluap1WT and Cluap1KO embryos demonstrate a significant decrease in expression of both Patched-1 and Gli1. Expression levels are relative to control peptidylprolyl isomerase A (PPIA). Bars represent mean fold expression, and error bars are ± SEM. Asterisks represent significant difference from control (**P < 0.01, Student’s t-test).