Figure 2

Normal ciliogenesis and correct non-GPCR membrane protein trafficking in tubby mutant. (A) The ependymal epithelia lining the lateral ventricles of WT mouse brain are multiciliated. These cilia are anchored by the ciliary rootlets. The ciliary axonemes are stained with an α-acetylated tubulin antibody (red) and the rootlets are highlighted by staining for rootletin (green). The upper image shows a schematic diagram and the lower image shows actual immunofluorescence. (B) In both WT and mutant tissues, ependymal epithelia appear to develop cilia of comparable length, abundance and organization. The ciliary rootlets (green) appear as a patch beneath the cilia as they are not resolved individually. (C) Neuronal primary cilia in the CA1 hippocampus and the paraventricular hypothalamus are visualized by staining for ACIII (green). Rootletin (red) serves as a marker for the ciliary base. In both regions, the appearance of ACIII staining pattern is similar between the WT and tubby mutant. Cell nuclei were counter stained by DAPI (blue).