Figure 3

OFT development is impaired in cbs mutants at E14.5. Serial 10-μm transverse sections of E14.5 wild type (B-E) and cbs/cbs (B-G) embryos were stained with H & E. They are presented from cranial to caudal, and the left side of the body is to the right, dorsal is at the top of each panel. A schematic reconstruction of the OFT is shown (A), with lines indicating the planes of the sections (B-G). In the schematic, the vertical axis has been exaggerated threefold in order to clearly display the complex outflow tract structure. For a description of the defects seen in the cbs/cbs mutants, please refer to the results section. Abbreviations (alphabetically arranged): Aa, ascending aorta; Ao, aorta; Arc, aortic arch; At, thoracic aorta; Bt, brachiocephalic trunk; CF, common foregut tube; Da, ductus arteriosus; E, esophagus; La, left atrium; Ljv, left internal jugular vein; Lpa, left pulmonary artery; Lsa, left subclavian artery; Ll, left lung; Lt, left thymus; Lv, left ventricle; Pa, common pulmonary artery; Pt, pulmonary trunk; PTA, persistent truncus arteriosus; Rc, right common carotid artery; Rpa, right pulmonary artery; Rsa, right subclavian artery; Rt, right thymus; Rv, right ventricle; T, trachea. Scale bars: (B-E) 0.5 mm, (F,G) 0.2 mm. cbs, cobblestone; E14.5, embryonic day 14.5; OFT, outflow tract.