Disassembly of cilia adhesion prior to cell division. MDCK cells stably expressing SmoYFP and engaged in cilia contacts were treated with hepatic growth factor to induce cell division and cell movements. (A) Z-stack images were recorded every 9.3 min. The panels on the left are the maximum projection of z slices 1-10 (the apical surface of the cell). The filled arrowheads indicate the base of the cilia; the empty arrowheads indicate absent cilia. The right panel is a single section through the nuclei of the cell layer, where the progress of cell division is clearly visible. While the images of these cells were being collected, the upper cell (cyan) resorbed its cilium and divided. In the last frame, the cilium of one of the new daughter cells is visible. (B) Montage of the maximum projection of z slices 1-10 showing the five time points that directly proceed nuclear envelop breakdown. The loss of the upper cilium is visible. Brightness and contrast were adjusted to make cilia more obvious.