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Figure 4 | Cilia

Figure 4

From: Live imaging of individual cell divisions in mouse neuroepithelium shows asymmetry in cilium formation and Sonic hedgehog response

Figure 4

Neural tube slice preparation for live imaging. (A) Unturned embryo E8.5 with first appearance of somite pairs. (B) Neural tube dissected in pre-warmed medium using a micro-knife. (C) Slice of the neural tube mounted ventral side down on the poly-L-lysine coated glass bottom dish. (D) Small amount of a mixture made from petroleum jelly and wax applied around the neural tube and gently covered by a narrow piece of glass coverslip. Bar is 300 μm (B).

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