Figure 4From: Live imaging of individual cell divisions in mouse neuroepithelium shows asymmetry in cilium formation and Sonic hedgehog responseNeural tube slice preparation for live imaging. (A) Unturned embryo E8.5 with first appearance of somite pairs. (B) Neural tube dissected in pre-warmed medium using a micro-knife. (C) Slice of the neural tube mounted ventral side down on the poly-L-lysine coated glass bottom dish. (D) Small amount of a mixture made from petroleum jelly and wax applied around the neural tube and gently covered by a narrow piece of glass coverslip. Bar is 300 μm (B).Back to article page