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Figure 5 | Cilia

Figure 5

From: Centrosomes in the zebrafish (Danio rerio): a review including the related basal body

Figure 5

Spindle organization and centrosome duplication defects in maternally mutant cellular atoll ( cea ) zebrafish embryos. (A) to (D') Embryos labeled with anti-α-tubulin antibody. (A) and (B') Fixed wild-type embryos. (C) and (D') Mutant embryos. Spindle organization is normal in the mutant embryos immediately prior to the first cell division (35 minutes postfertilization (pf) (C); compare with (A)) but is defective in a fraction of blastomeres in the next cleavage cycle (50 minutes pf (D) and (D'); compare with (B) and (B')). (E) to (H') Embryos labeled with anti-γ-tubulin antibody. (E) and (F') Fixed wild-type. (G) and (H') Mutant. Centrosome duplication appears normal immediately prior to the first cleavage division (35 minutes pf (G); compare with (E)) but is defective in a fraction of the blastomeres in the following cleavage cycle (50 minutes pf (H) and (H'); compare with (F) and (F')). Animal views: (B'), (D'), (F') and (H') are enlargements of the area indicated by the squares in (B), (D), (F) and (H), respectively. Reprinted with permission [71].

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