Figure 3
From: New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas
PCR assays to provide evidence for identifying the fla24 gene by reversion. A dCAPS marker shows reversion of fla24. A 303 bp PCR fragment was amplified in wild-type (FLA24), fla24, and three fla24 revertants (D11, D12, and D42b). The amplified fragment was subjected to AlwNI digestion, which generates three fragments of 51, 120, and 133 bps if the enzyme site is present in the PCR product as in wild-type and the true revertant (D11), or two fragments of 51 and 273 bps in the fla24 mutant and pseudorevertants (D12 and D42b).