Figure 5
From: New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas
Localization of D1bLIC in wild-type, fla24 , and fla24 revertants at 21°C and 32°C. D1bLIC staining is shown in green (first column) and the flagella are labeled with acetylated α-tubulin (red, second column). Merged images of both staining are shown in the third column. Overexposed D1bLIC signals are shown in the forth column to show the localization of D1bLIC in the flagella. Cells were obtained from 21°C and various time points at 32°C, as indicated. (A) Wild-type and fla24 cells. (B) fla24 revertants. (C) Twenty micrograms of whole cell protein extract were isolated from wild-type (FLA24) and fla24 cells at both 21°C and 32°C, and probed with the D1bLIC antibody. The membrane was then stripped and reprobed with α-tubulin to normalize the loading.