New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas

Lin, Huawen; Nauman, Nicholas P; Albee, Alison J; Hsu, Silas; Dutcher, Susan K

doi:10.1186/2046-2530-2-14

Cilia

Table 1 Phenotypes of intraflagellar transport (IFT) mutants and depletion

From: New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas

Conditional IFT mutants in *Chlamydomonas*
Gene	IFT phenotype at 21°C	Protein	Other names	Reference(s)
FLA1	Reduced anterograde velocity and train number	Kinesin-2 motor subunit	fla8-2	[8, 11, 24, 25]
FLA2	Reduced retrograde velocity and train number	Unknown		[24, 25, 28]
FLA3	Reduced frequency of anterograde trains	KAP subunit of kinesin-2		[7, 11, 24, 25]
FLA4	Wild-type	Unknown		[24, 25]
FLA5	Wild-type	Unknown		[11, 24, 25]
FLA6	Not tested	No longer extant		[24, 25]
FLA7	Not tested	Kinesin-2 motor subunit	fla10-14	[8, 24, 25, 29]
FLA8	Anterograde	Kinesin-2 motor subunit	fla8-1	[8, 25, 30]
FLA9	Wild-type	IFT81		[25], (unpublished data)
FLA10	Reduced anterograde velocity and train number	Kinesin-2 motor subunit	fla10-1	[3, 25, 30]
FLA11	Reduced retrograde velocity and train number	IFT172	fla11-1	[11, 25, 26]
FLA12	Faster anterograde and retrograde velocities	Unknown		[11, 29]
FLA13	Not tested	Unknown		[11, 29]
FLA15	Reduced retrograde velocity and train number	IFT139		[11, 31]
FLA16	Reduced retrograde velocity and train number	IFT144	fla17-2	[11, 31]
FLA17	Reduced retrograde velocity and train number	IFT144		[11, 31]
FLA18	Reduced anterograde velocity and train number	Kinesin-2 motor	fla10-16	[11], this report
FLA21	Wild-type	Unknown		[11]
FLA24	Reduced retrograde velocity and train number	DHC1b		[11], this report
FLA27	Decreased anterograde train number with wild-type velocity	Unknown		[11]
FLA28	Reduced anterograde velocity and train number	Unknown		[11]
DHC1b	Reduced retrograde velocity and train number	Cytoplasmic dynein	dhc1b ^ts ; dhcb1-3	[11, 27, 32]
Nonconditional IFT mutants in *Chlamydomonas*
Gene	Phenotype	Protein
FLA8	Aflagellate	Kinesin-2 motor subunit	fla8-3	[30]
FLA10	Aflagellate	Kinesin-2 motor subunit	fla10-2	[33]
FLA14	Short flagella	LC8		[34]
IFT46	Short, paralyzed flagella	IFT46		[18, 35, 36]
BLD1	Aflagellate	IFT52		[37, 38]
IFT80	Aflagellate	IFT80		[30]
IFT88	Very short flagella	IFT88		[36, 39, 40]
IFT121	Aflagellate	IFT121		[41]
IFT122	Aflagellate	IFT122
DHC1b/SPT1	Very short flagella	DHC1b	dhcb1; spt1-1; spt1-2	[9, 10]
D1bLIC	Variable flagellar length	D1bLIC	D1blic-1	[42]
RNAi depletion IFT strains in *Chlamydomonas*
IFT27	Short flagella	Rab-like		[43]
IFT70	Short flagella	IFT70/Dyf1		[44]

IFT, intraflagellar transport; KAP, kinesin-associated protein.

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ISSN: 2046-2530

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