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Table 1 Phenotypes of intraflagellar transport (IFT) mutants and depletion

From: New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas

Conditional IFT mutants in Chlamydomonas
Gene IFT phenotype at 21°C Protein Other names Reference(s)
FLA1 Reduced anterograde velocity and train number Kinesin-2 motor subunit fla8-2 [8, 11, 24, 25]
FLA2 Reduced retrograde velocity and train number Unknown   [24, 25, 28]
FLA3 Reduced frequency of anterograde trains KAP subunit of kinesin-2   [7, 11, 24, 25]
FLA4 Wild-type Unknown   [24, 25]
FLA5 Wild-type Unknown   [11, 24, 25]
FLA6 Not tested No longer extant   [24, 25]
FLA7 Not tested Kinesin-2 motor subunit fla10-14 [8, 24, 25, 29]
FLA8 Anterograde Kinesin-2 motor subunit fla8-1 [8, 25, 30]
FLA9 Wild-type IFT81   [25], (unpublished data)
FLA10 Reduced anterograde velocity and train number Kinesin-2 motor subunit fla10-1 [3, 25, 30]
FLA11 Reduced retrograde velocity and train number IFT172 fla11-1 [11, 25, 26]
FLA12 Faster anterograde and retrograde velocities Unknown   [11, 29]
FLA13 Not tested Unknown   [11, 29]
FLA15 Reduced retrograde velocity and train number IFT139   [11, 31]
FLA16 Reduced retrograde velocity and train number IFT144 fla17-2 [11, 31]
FLA17 Reduced retrograde velocity and train number IFT144   [11, 31]
FLA18 Reduced anterograde velocity and train number Kinesin-2 motor fla10-16 [11], this report
FLA21 Wild-type Unknown   [11]
FLA24 Reduced retrograde velocity and train number DHC1b   [11], this report
FLA27 Decreased anterograde train number with wild-type velocity Unknown   [11]
FLA28 Reduced anterograde velocity and train number Unknown   [11]
DHC1b Reduced retrograde velocity and train number Cytoplasmic dynein dhc1b ts ; dhcb1-3 [11, 27, 32]
Nonconditional IFT mutants in Chlamydomonas
Gene Phenotype Protein   
FLA8 Aflagellate Kinesin-2 motor subunit fla8-3 [30]
FLA10 Aflagellate Kinesin-2 motor subunit fla10-2 [33]
FLA14 Short flagella LC8   [34]
IFT46 Short, paralyzed flagella IFT46   [18, 35, 36]
BLD1 Aflagellate IFT52   [37, 38]
IFT80 Aflagellate IFT80   [30]
IFT88 Very short flagella IFT88   [36, 39, 40]
IFT121 Aflagellate IFT121   [41]
IFT122 Aflagellate IFT122   
DHC1b/SPT1 Very short flagella DHC1b dhcb1; spt1-1; spt1-2 [9, 10]
D1bLIC Variable flagellar length D1bLIC D1blic-1 [42]
RNAi depletion IFT strains in Chlamydomonas
IFT27 Short flagella Rab-like   [43]
IFT70 Short flagella IFT70/Dyf1   [44]
  1. IFT, intraflagellar transport; KAP, kinesin-associated protein.