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Table 3 Primers for mapping of fla18 in crosses with CC-1952 (S1C5)

From: New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas

Primer name

Sequence F (5’ to 3’)

Sequence R (5’ to 3’)

Product size in fla18and restriction enzyme

Fla18:S1C5 parental to recombinant progeny

Ch17-0.5

GCA CAG CTG AAG CGC AAA AGG AAG C

CGT TTC TCG AAC TCA GCC ACT GT

HindIII 180 bp

11:9 Unlinked

LC5-2.32

GGA CGG TGG GTA TGC ATT AG

GCT GTC ACT ACG TGG TCT CG

MspI 203 bp

15:5 (20 mu)

Ch17-4.03

ATA TTA CGC CTC TCC GAC AAC AGC

CAG CTT CTT TGT GCG CTT GTA CTT

-271 bp

19:1 (5 mu)

Ch17-6.28

CAT CGA GCT GCT TGG AGG CCA GAT A

CGC TAT ACA CCA CAT AGC GTC GAG

-147 bp

16:4 (16 mu)

  1. Markers without a restriction enzyme generate size polymorphisms due to CA repeats. All reactions were performed with an annealing temperature of 53°C.