Skip to main content

Table 3 Primers for mapping of fla18 in crosses with CC-1952 (S1C5)

From: New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas

Primer name Sequence F (5’ to 3’) Sequence R (5’ to 3’) Product size in fla18and restriction enzyme Fla18:S1C5 parental to recombinant progeny
Ch17-0.5 GCA CAG CTG AAG CGC AAA AGG AAG C CGT TTC TCG AAC TCA GCC ACT GT HindIII 180 bp 11:9 Unlinked
LC5-2.32 GGA CGG TGG GTA TGC ATT AG GCT GTC ACT ACG TGG TCT CG MspI 203 bp 15:5 (20 mu)
Ch17-4.03 ATA TTA CGC CTC TCC GAC AAC AGC CAG CTT CTT TGT GCG CTT GTA CTT -271 bp 19:1 (5 mu)
Ch17-6.28 CAT CGA GCT GCT TGG AGG CCA GAT A CGC TAT ACA CCA CAT AGC GTC GAG -147 bp 16:4 (16 mu)
  1. Markers without a restriction enzyme generate size polymorphisms due to CA repeats. All reactions were performed with an annealing temperature of 53°C.