Skip to main content

Table 5 Reversion of fla24 allele provides evidence of causality

From: New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas

Strain name

AlwNI

NciI

Sequence for digestion (underline)a

Amino acid

Sequence

fla24

Not cut

Cut

CAGCTGCCGGG

Proline

CCG

Wild-type

Cut

Not cut

CAGCTGCTGGG

Leucine

CTG

D7

Cut

Not Cut

CAGCTGCTGGG

P to L

CCG to CTG

D9b

Cut

Not Cut

CAGCTGCTGGG

P to L

CCG to CTG

D11

Cut

Not Cut

CAGCTGCTGGG

P to L

CCG to CTG

D12

Not cut

Not Cut

CAGCTGTCGGG

P to S

CCG to TCG

D25b

Cut

Not Cut

CAGCTGCTGGG

P to L

CCG to CTG

D37

Cut

Not Cut

CAGCTGCTGGG

P to L

CCG to CTG

D42b

Not cut

Not Cut

CAGCTGCGGGG

P to R

CCG to CGG

D43

Not cut

Not Cut

CAGCTGTCGGG

P to S

CCG to TCG

D57

Cut

Not Cut

CAGCTGCTGGG

P to L

CCG to CTG

  1. aThe underlined nucleotides in the fourth column indicate the recognition site for NciI or AlwNI.