Skip to main content

Table 5 Reversion of fla24 allele provides evidence of causality

From: New mutations in flagellar motors identified by whole genome sequencing in Chlamydomonas

Strain name AlwNI NciI Sequence for digestion (underline)a Amino acid Sequence
fla24 Not cut Cut CAGCTGCCGGG Proline CCG
Wild-type Cut Not cut CAGCTGCTGGG Leucine CTG
D7 Cut Not Cut CAGCTGCTGGG P to L CCG to CTG
D9b Cut Not Cut CAGCTGCTGGG P to L CCG to CTG
D11 Cut Not Cut CAGCTGCTGGG P to L CCG to CTG
D12 Not cut Not Cut CAGCTGTCGGG P to S CCG to TCG
D25b Cut Not Cut CAGCTGCTGGG P to L CCG to CTG
D37 Cut Not Cut CAGCTGCTGGG P to L CCG to CTG
D42b Not cut Not Cut CAGCTGCGGGG P to R CCG to CGG
D43 Not cut Not Cut CAGCTGTCGGG P to S CCG to TCG
D57 Cut Not Cut CAGCTGCTGGG P to L CCG to CTG
  1. aThe underlined nucleotides in the fourth column indicate the recognition site for NciI or AlwNI.