Figure 2

Hydin is incorporated early during CP assembly. WT cells before (pre) and at various time points (0, 10, 20 min) after deflagellation were analyzed by immunofluorescence microscopy using anti-hydin (a, d, g, j) and anti-acetylated tubulin (b, e, h, k). Merged images are shown in c, f, i, and l. Arrowheads in g: short flagella containing hydin. Bar = 5 μm.