Figure 3

Distribution of hydin during CP assembly. Gametes (a–c) and zygotes (d–l) from a mating of the CP mutant pf19 with WT (CC124) were analyzed by immunofluorescence microscopy using anti-acetylated α-tubulin and anti-hydin, as indicated. Arrows mark flagella of pf19 gametes (a) or flagella derived from pf19 in quadriflagellated zygotes (d, g, and j). Arrowheads in b and e: flagella largely lacking hydin, indicating the absence of a CP. Arrowheads in h and k: hydin accumulation in subdistal regions of flagella derived from the CP mutant. Note that the accumulation occurs symmetrically in the two flagella of a given zygote. Bar = 10 μm.