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Figure 6 | Cilia

Figure 6

From: Flagellar central pair assembly in Chlamydomonas reinhardtii

Figure 6

Distribution of PF6 during de novo CP assembly. Gametes and zygotes from a mating of pf15 cells with WT cells expressing triple HA-tagged α-tubulin were analyzed by immunofluorescence microscopy using anti-α-tubulin (a1–e1), anti-HA (a2–e2), and anti-PF6 (a3–e3). Merged images are shown in a4–e4. Arrows in a1–e1: flagella of pf15 gametes (a1) or zygotic flagella derived from pf15 (b1–e1). Filled arrowheads in c2–e2: developing CP as detected with anti-HA. Open arrowheads in c–e: incorporation of HA-tubulin at the distal ends of flagella derived from pf15. Arrows in a3 and b3: PF6 is present in the proximal regions of flagella of pf15 gametes and of flagella derived from pf15 in early quadriflagellates. Double arrowheads: position of PF6 during CP development. Inserts in c4: PF6 (red) is only present in the proximal regions of the assembling CP; accumulation of HA-tubulin (green) at the distal tip represents tubulin turnover in the outer doublets. Arrowheads: PF6-deficient regions of the newly formed CP. Bar = 10 μm.

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