Figure 7

Distribution of PF6 during repair of pf6 mutant flagella. Gametes (a) and zygotes (b–f) from matings of pf6 with WT (a–d), pf6 with pf15 (f), and, as a control for antibody specificity, pf6 with pf6 (e) were labeled with antibodies to acetylated α-tubulin and PF6. Merged images, mostly counterstained with DAPI to visualize the nuclei, are shown in the third column. Arrowheads in b2 and c2: incorporation of PF6 near the tip of zygotic flagella derived from pf6. In f, note the strikingly different distribution of PF6 in the lower pair of flagella derived from the CP-deficient strain pf15 vs. the upper pair of flagella derived from the PF6-deficient strain. Bar = 10 μm.