Figure 9

IFT proteins accumulate in CP-deficient flagella. (A) Western blot probing isolated flagella (FLA), axonemes (AXO), and the membrane + matrix fraction (M + M) of WT (g1) and the CP-deficient mutants pf18 and pf19 with the antibodies indicated. Similar results were obtained for pf15 (not shown). (B) Immunofluorescence microscopy of WT and pf15 cells extracted with detergent and fixed with formaldehyde either simultaneously (top) or sequentially (bottom) and then stained with antibodies to acetylated tubulin and IFT20. Note retention of IFT20 in pf15 but not WT axonemes extracted before fixation.