Figure 1
From: Interleukin-1β sequesters hypoxia inducible factor 2α to the primary cilium
Interleukin-1β induces dose dependent and reversible cilia elongation. Confluent monolayers were treated with IL-1β before immunofluorescent staining of cilia. (A) Staining of cilia with anti-acetylated-α- tubulin and (B) concurrent anti-arl13b staining with 24 h IL-1β (10 ng.mL-1) treatment (scale =5 μm). (C) Bovine chondrocyte cilia length-IL-1 dose response at 24 h and (D) elongation time course at 10 ng.mL-1. (E) Ciliary elongation in human articular chondrocytes exposed to IL-1β (10 ng.mL-1, 24 h). (F) Reversibility of cilia elongation induced by IL-1β (10 ng.mL-1). (G) Inhibition of IL-1β induced-elongation with HDAC and ROCK inhibitors.