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Figure 2 | Cilia

Figure 2

From: Interleukin-1β sequesters hypoxia inducible factor 2α to the primary cilium

Figure 2

Interleukin-1β increased HIF-2α expression. Confluent preparations were treated with cytokines and total protein isolated. (A) Western blot analysis for HIFα expression during IL-1-treatment (10 ng.mL-1) time course. (B/C) Quantitative analysis of relative HIFα expression normalised to untreated control and β-tubulin. (D) Cilia length, as determined from immunofluorescent staining, with concurrent IL-1 and Oncostatin-M treatment, both at 10 ng.mL-1. (E) Western blot analysis of HIF2α expression with concurrent IL-1 and Oncostatin-M treatments.

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