Figure 4

Prolyl hydroxylase inhibition and hypoxia increases cilia length and is associated with accumulation of HIF-2α at the cilium. (A) Immunofluorescent staining with anti-acetylated α tubulin with 24 h DMOG (10 μM) treatment (scale =5 μm). (B) Primary cilia length with prolyl hydroxylase inhibition. (C) Effect of 24 h hypoxia on primary cilia length. (D/E) Western blot analysis of HIFα expression after 24 h. (F) Immunofluorescent staining with anti-acetylated tubulin (red) and anti HIF-2α (green) in cells treated with DMOG. (G) Graphical summary of cilia localisation data for cells treated with DMOG, n above individual columns.