Figure 2

Absence of cellular defects in mutant lungs. Immunohistochemical analysis of cell-specific markers in wild type (A,D,G,J), nm1054 (B,E,H,K), and bgh (C,F,I,L) lungs. (A–C) Acetylated tubulin, a marker of epithelial cilia showing a normal appearance and distribution of cilia in both mutants. (D–F) CCSP, a marker of Clara cells. (G–I) T1α, a marker of alveolar type I cells. (J–L) Prosurfactant protein B, a marker of alveolar type II cells and Clara cells. Arrowheads indicate the stained epithelial (A–C), Clara (D–F), alveolar type I (G–I), and alveolar type II (J–L) cells. Scale bars represent 100 μm.