Figure 1From: Reduced cilia frequencies in human renal cell carcinomas versus neighboring parenchymal tissueImmunofluorescent analysis of cilia in renal tissues. (A) Sections (4 μm) of renal parenchymal tissues and tumor tissues were stained with DAPI, acetylated-α-tubulin (Ac-tub) and pericentrin (PCNT) to mark cell nuclei and cilia. Presented images are maximal projections of confocal images of typical parenchymal tissue and a representative ccRCC. Scale bars 20 μm. (B) Normalized cilia frequencies of renal tumors, shown are paired quantifications of n = 20 samples. The plot compares the two cilia quantification methodologies described; data was obtained by immunofluorescent (IF) confocal image acquisition or scoring of immunohistochemical (IHC) stained sections. Statistics were determined by performing a paired t-test at a 95% confidence interval.Back to article page