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Figure 2 | Cilia

Figure 2

From: An inducible CiliaGFP mouse model for in vivo visualization and analysis of cilia in live tissue

Figure 2

Analysis of Sstr3::GFP expression in the brain. (A-C) Images from ex vivo whole mount brains. Sstr3::GFP signal (green) in (A) hippocampal primary cilia, (B) ependymal motile cilia and (C) choroid plexus cilia. (D-F) Sstr3:GFP co-localization with known ciliary markers. (D) Motile cilia of the lateral ventricle, (E) pituitary primary cilia, and (F) hindbrain primary cilia. Cilia were immunolabeled (red) with either acetylated α-tubulin (AcetTub) in D and E or adenylate cyclase III (ACIII). Nuclei were stained with Hoechst (blue). Arrows indicate cilia that are double-labeled and arrowheads indicate cilia that are GFP negative in agreement with mosaic EIIa Cre activity.

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