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Figure 3 | Cilia

Figure 3

From: An inducible CiliaGFP mouse model for in vivo visualization and analysis of cilia in live tissue

Figure 3

Sstr3::GFP labeled cilia in the kidney. Sstr3::GFP signal (green) in (A) fixed cryosections of the kidney, (B) isolated EIIa Cre; CiliaGFPtubules, (C) in situ in the proximal tubule of an anesthetized mouse. Immunofluorescence staining of (D) isolated tubules and (E) kidney sections and Sstr3:GFP localization. Acetylated α-tubulin (red) labeling was used to validate cilia localization of the Sstr3::GFP fusion protein. Nuclei are labeled with Hoescht (blue). Arrows indicate examples of cilia that are double-labeled.

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