Skip to main content
Figure 6 | Cilia

Figure 6

From: An inducible CiliaGFP mouse model for in vivo visualization and analysis of cilia in live tissue

Figure 6

Cell type specific labeling of cilia using Cre mediated activation in the CiliaGFPmouse. (A) Control section of the dentate gyrus in a POMC Cre; CiliaGFP mouse showing lack of green Sstr3::GFP containing cilia. Cilia are identified by immunofluorescence analysis with ACIII (red). (B-D) Sstr3::GFP cilia (green) are seen in the area of the arcuate nuclei (ARC) of the hypothalamus surrounding the third ventricle (III) in POMC Cre; CiliaGFP mice. Cilia in the ARC were identified by immunofluorescence analysis with ACIII (red) in (C and D). Examples of dual labeled ACIII (red) and Sstr3::GFP (green) positive cilia are indicated by arrows, an example of a cilium labeled with ACIII but not Sstr3::GFP that is likely on non-POMC neurons is indicated by an arrowhead. (E) Sstr3::GFP expression in cilia on pancreatic islet cells from a Pdx1 CreER; CiliaGFP mouse after tamoxifen induction. Cilia are labeled with (E) acetylated α-tubulin (Acet Tub) in red. Many cilia were positive for both Sstr3::GFP and acetylated α-tubulin (arrows) while other cilia did not possess Sstr3::GFP and are likely non-β-cells (arrowhead).

Back to article page