Figure 6From: An inducible CiliaGFP mouse model for in vivo visualization and analysis of cilia in live tissueCell type specific labeling of cilia using Cre mediated activation in the CiliaGFPmouse. (A) Control section of the dentate gyrus in a POMC Cre; CiliaGFP mouse showing lack of green Sstr3::GFP containing cilia. Cilia are identified by immunofluorescence analysis with ACIII (red). (B-D) Sstr3::GFP cilia (green) are seen in the area of the arcuate nuclei (ARC) of the hypothalamus surrounding the third ventricle (III) in POMC Cre; CiliaGFP mice. Cilia in the ARC were identified by immunofluorescence analysis with ACIII (red) in (C and D). Examples of dual labeled ACIII (red) and Sstr3::GFP (green) positive cilia are indicated by arrows, an example of a cilium labeled with ACIII but not Sstr3::GFP that is likely on non-POMC neurons is indicated by an arrowhead. (E) Sstr3::GFP expression in cilia on pancreatic islet cells from a Pdx1 CreER; CiliaGFP mouse after tamoxifen induction. Cilia are labeled with (E) acetylated α-tubulin (Acet Tub) in red. Many cilia were positive for both Sstr3::GFP and acetylated α-tubulin (arrows) while other cilia did not possess Sstr3::GFP and are likely non-β-cells (arrowhead).Back to article page