Figure 7
From: An inducible CiliaGFP mouse model for in vivo visualization and analysis of cilia in live tissue
Sstr3::GFP localization in testes and sperm. (A) Sstr3::GFP localization in the mouse testes. Sstr3::GFP signal (green) can be seen in the sperm flagella in the center of the seminiferous tubules of CiliaGFP-ON mice (upper panels), which are also positive for acetylated α-tubulin (red). Sstr3::GFP signal is absent in wild-type mouse testes (lower panels). (B) Sstr3::GFP signal is localized to the flagellum of CiliaGFP mouse sperm but no signal is detected in wild-type mouse sperm. The arrow points to the example of a small subset of sperm where Sstr3::GFP appeared to be restricted to only the mid-piece of the flagellum. Nuclei are counterstained with Hoescht (blue).