Localization of FLAG-MKS3p. (A) Cells expressing FLAG (control) orFLAG-MKS3 were immunostained with anti-centrin (red) andanti-FLAG (green). Representative cells are shown. The oral grove (mouth) isindicated by yellow arrows. (B) FLAG staining in the test cellexpressing FLAG-MKS3 in (A) shows a punctatepattern at each basal body with partial colocalization. (C) Thisstaining is above the plane of the basal body (see also Additional file 4: Figure S4 for Western blots and Additional file 5: Movie S1 for individual Z sections). Images are stacksof Z sections, with thicknesses of 10 to 15 μm (A) and(B) or 4 μm (C). Scale bars are15 μm (A), 5 μm (B) and 2 μm(C).