Disruptions of the cortical units correspond to the areas of basal bodymisalignment. Control (A) and MKS3-depletedcells (B) and (C) were stained using anti-2F12 tovisualize the cortical ridges. These images show the dorsal surface and arestacks of Z sections approximately 10 μm thick. The bright greenimmunofluorescence spots indicate the oral grooves. The control cell in(A) shows the high degree of organization of the outer latticeacross the cell surface. The presence of a basal body in each unit can be seenin the enlarged area (A(a), yellow box) and the traced imagebelow (black dot inside the hexagonal unit). The MKS3-depleted cellsillustrate insertion of several cortical units forming an abbreviated kinety(B(b), yellow arrows) or a disruption type caused by theinsertion of multiple cortical units, resulting in the formation of a cluster(C(c), yellow arrows). The contractile vacuole pores areindicated by gray arrows. Scale bars: 10 μm.