Figure 5

Microtubule ribbons have an altered orientation in MKS3 depleted cells. IFT88-depleted cells(A) and MKS3-depleted cells (B) cells werestained using anti-centrin basal bodies (red) and anti-tubulin microtubulerootlets (green). Images are stacks of Z sections approximately 10 μmthick. All images show the anterior dorsal surface. Areas in(A) and (B) (yellow box) have been enlarged, andbelow each is a tracing to depict the basal body (red) and the emanatingrootlets (black). The rootlets are organized into the basal body rows(kineties) at 5 o’clock orientation (transverse microtubules (TMs)) and 7o’clock orientation (postciliary microtubules (PCMs)) relative to thebasal body. In the disrupted region of the MKS3-depleted cell(B), the microtubule rootlets are present and maintain the anglebetween them (C), but do not maintain the regular orientation along theanteroposterior cell axis as shown in the control cell. Scale bars:10 μm. The angles between the TM and the PCM were measured(C), and these angles were maintained in the MKS3-depletedcells (35 cells from both test and control cells). Error barsare ± SD.