Chaotic orientation of the striated rootlet in MKS3-depleted cells. Control cells(A) and MKS3-depleted cells (B) werestained with anti-Glu-α-tubulin (red; basal bodies) and anti-kinetodesmalfibers (green; striated rootlets). Yellow arrows in (A) and(B) indicate the contractile vacuoles on the dorsal surface ofthese cells. Striated rootlets (SRs) project from the basal bodies. In twobasal body units, SRs project only from the posterior basal body (a; dottedyellow arrows). SRs project toward the anterior of the cell in a highlyorganized manner along the kinety. Cells depleted of MKS3 depictchaotic organization of the SRs, which project in every direction (b andb′). Scale bar: 10 μm.