Figure 6

Comparison of wildtype (WT) and mutant BBS5-NT-GFP expression and localisation in HEK293 cells. Confocal fluorescent images (overlay, plus individual fluorescent channels respectively) from WT BBS5-NT-GFP (A,B) and mutant (Mut) BBS5-NT-GFP (C,D) are shown, together with pericentrin antibody (red) to localise centrosomes. The stain 4'-6-diamidino-2-phenylindole (DAPI) (blue) is used to stain nuclei. (A) WT BBS5-NT-GFP colocalises with pericentrin at centrosomes. There is some diffuse cytoplasmic and intranuclear localisation of WT BBS5-NT-GFP. (B) WT BBS5-NT-GFP colocalises with pericentrin at the centrosomes in a cell undergoing mitosis. (C,D) Mut BBS5-NT-GFP is distributed diffusely throughout the cell and fails to colocalise with the centrosomal marker pericentrin. Scale bar 10 μm.