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Figure 11 | Cilia

Figure 11

From: Mitochondrial fusion protein MFN2 interacts with the mitostatin-related protein MNS1 required for mouse sperm flagellar structure and function

Figure 11

SDS-PAGE and immunoblot of detergent extracted spermatogenic and sperm cells probed against anti-MFN2 and anti-MNS1. Immunoblot with anti-MFN2 (top) or anti-MNS1 (bottom) antibodies to analyze (left panels): spermatogenic cells, Triton X-100 soluble and insoluble spermatogenic cell proteins, and Triton X-100/S-EDTA soluble and insoluble spermatogenic cell proteins; and (right panels): cauda epididymal sperm, SDS-insoluble head and tail proteins, Triton X-100 soluble and insoluble whole sperm proteins, and Triton X-100/S-EDTA soluble and insoluble whole sperm proteins. MFN2 was present in cauda epididymal sperm and in the tail structures of the whole sperm. MNS1 was present as closely migrating bands in cauda epididymal sperm and spermatogenic cells. MNS1 was also present in detergent-resistant structures of the sperm and spermatogenic cells. It was seen more abundantly in the tail structures of the whole sperm versus the head. Cd: cauda epididymal sperm, MGC: mixed germ cells, Sup: supernatant, P: pellet. Numbers on the left of the panels indicate the molecular weights of standard proteins (×10-3). Asterisk (*) indicates expected size (MFN2: M r of 80 and MNS1: M r of 60) of protein.

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