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Table 1 List of primer sets used for collection of experimental data

From: Mitochondrial fusion protein MFN2 interacts with the mitostatin-related protein MNS1 required for mouse sperm flagellar structure and function

Primer name Application Forward primer (5’-3’) Reverse primer (5’-3’)
Mfn2 RT-PCR GCC AGC TTC CTT GAA GAC AC GCA GAA CTT TGT CCC AGA GC
Mns1 RT-PCR CAG TTC CTC GCA GAC AAA CA GGC CTT GCT ATG AAG ACT CG
Mitostatin RT-PCR GAC AAA GGC AGA GGC TTG AC CAA GCC ATA ACA ACC CGA CT
Gapdh RT-PCR GGT GAA GGT CGG TGT GAA CG CTC GCT CCT GGA AGA TGG TG
Mfn2-qPCR1 1 qRT-PCR ATG TTA CCA CGG AGC TGG AC AAC TGC TTC TCC GTC TGC AT
Mfn2-qPCR2 2 qRT-PCR CTA TGA GCG ACT GAC CTG GA CAC TGG CGT ATT CCA CAA AC
Mns1-qPCR1 1 qRT-PCR CCT CAA AGA ACA TGC TGC AA GGC CTT GCT ATG AAG ACT CG
Mns1-qPCR2 2 qRT-PCR AAG CAG CTG GAG GAA ACA CT TTG ATG ATC TCT GCC TGC TC
Gapdh-qPCR qRT-PCR AAC TTT GGC ATT GTG GAA GG GGA TGC AGG GAT GAT GTT CT
Ribosomal Protein S16 qRT-PCR AGA TGA TCG AGC CGC GC GCT ACC AGG GCC TTT GAG ATC GA