Figure 5
Endogenous TRAPPC8 localizes to the centrosome/basal body. RPE cells were serum-starved for 24Â h, fixed with methanol (upper two panels) or PFA (lower panel) and stained with rabbit polyclonal antibody against TRAPPC8 (green) and mouse monoclonal antibody against p150Glued, rat monoclonal antibody against EB3 or mouse monoclonal antibody against acetylated tubulin (Ac tub), as indicated (red). DNA was stained with DAPI. Arrowheads and asterisks point to the centrosomes/basal bodies. Closed arrow indicates a primary cilium.