Figure 5

Characterization of human breast cancer samples for expression of ciliogenesis genes. (A) Heat map displays the relative gene expression change seen in basal-like cancer samples compared to normal breast tissue. Each column represents a breast cancer tissue sample (sample name listed on top) and each row represents the relative expression results of a different ciliary gene (gene names are to the right). Overexpression is depicted in red, and underexpression is depicted in green. (B) The boxplots represent distribution of expression values for individual genes comparing breast cancer tissue samples to the normal tissues samples. The ciliary-associated genes shown in boxplots are those that were found to be statistically significant by ANOVA (Analysis of Variance) between normal and basal-like cancer samples with adjusted *P value <0.05. The log odds that the gene is differentially expressed are provided by B-statistics and B >3.0 was used as a cutoff for significantly changing genes. (C) Images show staining of normal breast reduction mammoplaties or breast cancer tissue with secondary antibody alone or with an antibody recognizing DYNC2H1 (red). Inset in normal breast tissue shows that tissue was also co-stained for antibodies to identify cilia (acetylated tubulin, green; γ-tubulin, white) and analyzed for co-localization in the same cell. The arrow points to a cilium with a pool of DYNC2H1 at the base.